METAP2 ELISA Kit

Catalog No. ABIN5656865

Product Details METAP2 ELISA Kit

Target: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Methionyl Aminopeptidase 2 (METAP2). No significant cross-reactivity or interference between Methionyl Aminopeptidase 2 (METAP2) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionyl Aminopeptidase 2 (METAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionyl Aminopeptidase 2 (METAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionyl Aminopeptidase 2 (METAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionyl Aminopeptidase 2 (METAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionyl Aminopeptidase 2 (METAP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionyl Aminopeptidase 2 (METAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for METAP2

Target: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Alternative Name: Methionyl Aminopeptidase 2 (METAP2 Products)

Synonyms: metap2 ELISA Kit, MGC53792 ELISA Kit, METAP2 ELISA Kit, GB13458 ELISA Kit, DDBDRAFT_0190923 ELISA Kit, DDBDRAFT_0304991 ELISA Kit, DDB_0190923 ELISA Kit, DDB_0304991 ELISA Kit, 4930584B20Rik ELISA Kit, A930035J23Rik ELISA Kit, AI047573 ELISA Kit, AL024412 ELISA Kit, AU014659 ELISA Kit, Amp2 ELISA Kit, Mnpep ELISA Kit, p67 ELISA Kit, p67eIF2 ELISA Kit, MAP2 ELISA Kit, MNPEP ELISA Kit, wu:fb98h06 ELISA Kit, zgc:66250 ELISA Kit, methionyl aminopeptidase 2 L homeolog ELISA Kit, methionyl aminopeptidase 2 ELISA Kit, methionine aminopeptidase 2 ELISA Kit, methionine aminopeptidase ELISA Kit, methionyl aminopeptidase 2 S homeolog ELISA Kit, methionyl aminopeptidase 2b ELISA Kit, metap2.L ELISA Kit, METAP2 ELISA Kit, metap2 ELISA Kit, LOC551771 ELISA Kit, CAALFM_C604080WA ELISA Kit, Metap2 ELISA Kit, metap2.S ELISA Kit, metap2b ELISA Kit

Background:

Gene Name: Methionyl Aminopeptidase 2

Gene Aliases: MNPEP, p67, p67eIF2, Peptidase M, Initiation factor 2-associated 67 kDa glycoprotein

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling