MAT2A ELISA Kit

Catalog No. ABIN5656862

Product Details MAT2A ELISA Kit

Target: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Methionine Adenosyltransferase II Alpha (MAT2a). No significant cross-reactivity or interference between Methionine Adenosyltransferase II Alpha (MAT2a) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionine Adenosyltransferase II Alpha (MAT2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionine Adenosyltransferase II Alpha (MAT2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionine Adenosyltransferase II Alpha (MAT2a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionine Adenosyltransferase II Alpha (MAT2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MAT2A

Target: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Alternative Name: Methionine Adenosyltransferase II Alpha (MAT2A Products)

Synonyms: MATA2 ELISA Kit, MATII ELISA Kit, SAMS2 ELISA Kit, Sams2 ELISA Kit, D630045P18Rik ELISA Kit, fd12a12 ELISA Kit, mat2a ELISA Kit, si:ch73-340n8.1 ELISA Kit, wu:fb95e01 ELISA Kit, wu:fd12a12 ELISA Kit, m(2)21ab ELISA Kit, MGC76253 ELISA Kit, MGC79598 ELISA Kit, zgc:110847 ELISA Kit, methionine adenosyltransferase 2A ELISA Kit, methionine adenosyltransferase II, alpha ELISA Kit, methionine adenosyltransferase II, alpha a ELISA Kit, methionine adenosyltransferase II, alpha L homeolog ELISA Kit, methionine adenosyltransferase II, alpha b ELISA Kit, MAT2A ELISA Kit, Mat2a ELISA Kit, mat2aa ELISA Kit, mat2a.L ELISA Kit, mat2a ELISA Kit, mat2ab ELISA Kit

Background:

Gene Name: Methionine Adenosyltransferase II Alpha

Gene Aliases: SAMS2, MATA2, AMS2, MATII, S-adenosylmethionine synthase isoform type-2

Gene ID: 4144

UniProt: P31153

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process