MAT1A ELISA Kit

Catalog No. ABIN5656859

Product Details MAT1A ELISA Kit

Target: MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Methionine Adenosyltransferase I Alpha (MAT1a). No significant cross-reactivity or interference between Methionine Adenosyltransferase I Alpha (MAT1a) and analogues was observed.

Sensitivity: 0.28 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionine Adenosyltransferase I Alpha (MAT1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionine Adenosyltransferase I Alpha (MAT1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionine Adenosyltransferase I Alpha (MAT1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionine Adenosyltransferase I Alpha (MAT1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionine Adenosyltransferase I Alpha (MAT1a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionine Adenosyltransferase I Alpha (MAT1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MAT1A

Target: MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))

Alternative Name: Methionine Adenosyltransferase I Alpha (MAT1A Products)

Synonyms: MAT ELISA Kit, MATA1 ELISA Kit, SAMS ELISA Kit, SAMS1 ELISA Kit, AdoMet ELISA Kit, SADE ELISA Kit, SAS ELISA Kit, AI046368 ELISA Kit, Ams ELISA Kit, wu:fi35e01 ELISA Kit, zgc:55442 ELISA Kit, methionine adenosyltransferase 1A ELISA Kit, methionine adenosyltransferase I, alpha ELISA Kit, MAT1A ELISA Kit, Mat1a ELISA Kit, mat1a ELISA Kit

Background:

Gene Name: Methionine Adenosyltransferase I Alpha

Gene Aliases: MAT, SAMS, MATA1, SAMS1, AMS1, S-Adenosylmethionine Synthetase, S-adenosylmethionine synthase isoform type-1

Pathways: Mitotic G1-G1/S Phases, M Phase, Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process