METRN ELISA Kit

Catalog No. ABIN5656854

Product Details METRN ELISA Kit

Target: METRN (Meteorin, Glial Cell Differentiation Regulator (METRN))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Meteorin (METRN). No significant cross-reactivity or interference between Meteorin (METRN) and analogues was observed.

Sensitivity: 5.6 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meteorin (METRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meteorin (METRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meteorin (METRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Meteorin (METRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meteorin (METRN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meteorin (METRN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for METRN

Target: METRN (Meteorin, Glial Cell Differentiation Regulator (METRN))

Alternative Name: Meteorin (METRN Products)

Synonyms: mtrn ELISA Kit, zgc:92132 ELISA Kit, wu:fb81a08 ELISA Kit, METRN ELISA Kit, C16orf23 ELISA Kit, c380A1.2 ELISA Kit, Hyrac ELISA Kit, RGD1306222 ELISA Kit, 1810034B16Rik ELISA Kit, meteorin, glial cell differentiation regulator ELISA Kit, meteorin ELISA Kit, METRN ELISA Kit, metrn ELISA Kit, Metrn ELISA Kit

Background:

Gene Name: Meteorin

Gene Aliases: C16orf23, RJD6, Glial Cell Differentiation Regulator

Gene ID: 70083

UniProt: Q8C1Q4