MANF ELISA Kit

Catalog No. ABIN5656833

Product Details MANF ELISA Kit

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). No significant cross-reactivity or interference between Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) and analogues was observed.

Sensitivity: 6.1 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF Products)

Synonyms: ARMET ELISA Kit, ARP ELISA Kit, 3230402M22Rik ELISA Kit, AA407711 ELISA Kit, AA408789 ELISA Kit, AA673178 ELISA Kit, Armet ELISA Kit, D17914 ELISA Kit, D18Mgi17 ELISA Kit, MANF ELISA Kit, RBM15B ELISA Kit, ARP-like ELISA Kit, CG7013 ELISA Kit, DmMANF ELISA Kit, Dmel\\CG7013 ELISA Kit, anon-EST:ParkEST188 ELISA Kit, arp ELISA Kit, armet ELISA Kit, im:6909481 ELISA Kit, zgc:153327 ELISA Kit, manf ELISA Kit, mesencephalic astrocyte derived neurotrophic factor ELISA Kit, mesencephalic astrocyte-derived neurotrophic factor ELISA Kit, RNA binding motif protein 15B ELISA Kit, Mesencephalic astrocyte-derived neurotrophic factor ELISA Kit, mesencephalic astrocyte-derived neurotrophic factor S homeolog ELISA Kit, MANF ELISA Kit, Manf ELISA Kit, RBM15B ELISA Kit, manf ELISA Kit, manf.S ELISA Kit

Background:

Gene Name: Mesencephalic Astrocyte Derived Neurotrophic Factor

Gene Aliases: ARMET, ARP, Arginine Rich Mutated In Early Stage Tumors, Arginine-rich protein

Gene ID: 7873

UniProt: P55145