MCAM CLIA Kit

Catalog No. ABIN5656812

Product Details MCAM CLIA Kit

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Melanoma Cell Adhesion Molecule (MCAM). No significant cross-reactivity or interference between Melanoma Cell Adhesion Molecule (MCAM) and analogues was observed.

Sensitivity: 0.03 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Melanoma Cell Adhesion Molecule (MCAM) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule (MCAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule (MCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: Melanoma Cell Adhesion Molecule (MCAM Products)

Synonyms: CD146 CLIA Kit, MUC18 CLIA Kit, Muc18 CLIA Kit, 1-gicerin CLIA Kit, AV025631 CLIA Kit, CD149 CLIA Kit, s-endo CLIA Kit, s-gicerin CLIA Kit, cd146 CLIA Kit, gicerin CLIA Kit, id:ibd3512 CLIA Kit, mcam CLIA Kit, MCAM CLIA Kit, melanoma cell adhesion molecule CLIA Kit, melanoma cell adhesion molecule b CLIA Kit, MCAM CLIA Kit, Mcam CLIA Kit, mcamb CLIA Kit

Background:

Gene Name: Melanoma Cell Adhesion Molecule

Gene Aliases: CD146, MUC18, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, S-endo 1 endothelial-associated antigen

Gene ID: 84004

UniProt: Q8R2Y2