NG2 ELISA Kit

Catalog No. ABIN5656809

Product Details NG2 ELISA Kit

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP). No significant cross-reactivity or interference between Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP Products)

Synonyms: HMW-MAA ELISA Kit, MCSP ELISA Kit, MCSPG ELISA Kit, MEL-CSPG ELISA Kit, MSK16 ELISA Kit, NG2 ELISA Kit, 4732461B14Rik ELISA Kit, AN2 ELISA Kit, Ng2 ELISA Kit, HSMCSGEN1 ELISA Kit, MCS ELISA Kit, chondroitin sulfate proteoglycan 4 ELISA Kit, sperm mitochondria associated cysteine rich protein ELISA Kit, CSPG4 ELISA Kit, Cspg4 ELISA Kit, SMCP ELISA Kit

Background:

Gene Name: Melanoma Associated Chondroitin Sulfate Proteoglycan

Gene Aliases: CSPG4, MSK16, HMW-MAA, MCSP, MCSPG, MEL-CSPG, NG2, Chondroitin Sulfate Proteoglycan 4, Melanoma chondroitin sulfate proteoglycan

Gene ID: 1464

UniProt: Q6UVK1

Pathways: Glycosaminoglycan Metabolic Process