MC5 Receptor CLIA Kit

Catalog No. ABIN5656805

Product Details MC5 Receptor CLIA Kit

Target: MC5 Receptor (MC5R) (Melanocortin 5 Receptor (MC5R))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Melanocortin 5 Receptor (MC5R). No significant cross-reactivity or interference between Melanocortin 5 Receptor (MC5R) and analogues was observed.

Sensitivity: 5.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Melanocortin 5 Receptor (MC5R). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Melanocortin 5 Receptor (MC5R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Melanocortin 5 Receptor (MC5R) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanocortin 5 Receptor (MC5R) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanocortin 5 Receptor (MC5R) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: MC5 Receptor (MC5R) (Melanocortin 5 Receptor (MC5R))

Alternative Name: Melanocortin 5 Receptor (MC5R Products)

Synonyms: MC2 CLIA Kit, MC5R CLIA Kit, MC5 CLIA Kit, mc5br CLIA Kit, mc5ra CLIA Kit, zMC5b CLIA Kit, zgc:194217 CLIA Kit, mc5ar CLIA Kit, mc5rb CLIA Kit, zMC5a CLIA Kit, melanocortin 5 receptor CLIA Kit, melanocortin 5b receptor CLIA Kit, melanocortin 5a receptor CLIA Kit, MC5R CLIA Kit, Mc5r CLIA Kit, mc5r CLIA Kit, mc5rb CLIA Kit, mc5ra CLIA Kit

Background:

Gene Name: Melanocortin 5 Receptor

Gene Aliases: MC-2

Pathways: cAMP Metabolic Process