MDM2 ELISA Kit

Catalog No. ABIN5656802

Product Details MDM2 ELISA Kit

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Mdm2 p53 Binding Protein Homolog (MDM2). No significant cross-reactivity or interference between Mdm2 p53 Binding Protein Homolog (MDM2) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mdm2 p53 Binding Protein Homolog (MDM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mdm2 p53 Binding Protein Homolog (MDM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mdm2 p53 Binding Protein Homolog (MDM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mdm2 p53 Binding Protein Homolog (MDM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mdm2 p53 Binding Protein Homolog (MDM2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mdm2 p53 Binding Protein Homolog (MDM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: Mdm2 p53 Binding Protein Homolog (MDM2 Products)

Synonyms: ACTFS ELISA Kit, HDMX ELISA Kit, hdm2 ELISA Kit, zgc:109834 ELISA Kit, 1700007J15Rik ELISA Kit, AA415488 ELISA Kit, Mdm-2 ELISA Kit, MDM2 proto-oncogene ELISA Kit, MDM2 proto-oncogene S homeolog ELISA Kit, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) ELISA Kit, MDM2 oncogene, E3 ubiquitin protein ligase ELISA Kit, transformed mouse 3T3 cell double minute 2 ELISA Kit, MDM2 ELISA Kit, mdm2.S ELISA Kit, Mdm2 ELISA Kit, mdm2 ELISA Kit

Background:

Gene Name: Mdm2 p53 Binding Protein Homolog

Gene Aliases: HDMX, hdm2, Mouse Double Minute 2,Human Homolog Of p53-Binding Protein, Oncoprotein Mdm2

Gene ID: 4193

UniProt: Q00987

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway