MMP14 ELISA Kit

Catalog No. ABIN5656731

Product Details MMP14 ELISA Kit

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 14 (MMP14). No significant cross-reactivity or interference between Matrix Metalloproteinase 14 (MMP14) and analogues was observed.

Sensitivity: 6.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 14 (MMP14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 14 (MMP14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 14 (MMP14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 14 (MMP14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 14 (MMP14) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 14 (MMP14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MMP14

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Alternative Name: Matrix Metalloproteinase 14 (MMP14 Products)

Synonyms: MMP1 ELISA Kit, MMP-14 ELISA Kit, MMP-X1 ELISA Kit, MT-MMP ELISA Kit, MT-MMP 1 ELISA Kit, MT1-MMP ELISA Kit, MT1MMP ELISA Kit, MTMMP1 ELISA Kit, mmp-x1 ELISA Kit, mt1-mmp ELISA Kit, mtmmp1 ELISA Kit, AI325305 ELISA Kit, MT-MMP-1 ELISA Kit, sabe ELISA Kit, Mt1-mmp ELISA Kit, MT-MMPbeta ELISA Kit, cb386 ELISA Kit, sb:cb386 ELISA Kit, wu:fb72b09 ELISA Kit, mmp14 ELISA Kit, matrix metallopeptidase 14 ELISA Kit, matrix metallopeptidase 14 (membrane-inserted) ELISA Kit, matrix metallopeptidase 14b (membrane-inserted) ELISA Kit, matrix metallopeptidase 14a (membrane-inserted) ELISA Kit, matrix metallopeptidase 14 L homeolog ELISA Kit, MMP14 ELISA Kit, mmp14 ELISA Kit, Mmp14 ELISA Kit, mmp14b ELISA Kit, mmp14a ELISA Kit, mmp14.L ELISA Kit

Background:

Gene Name: Matrix Metalloproteinase 14

Gene Aliases: MMP-X1, MT1-MMP, MTMMP1, Membrane Inserted, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase

Gene ID: 17387

UniProt: P53690

Pathways: Autophagy