MAN2B1 ELISA Kit

Catalog No. ABIN5656654

Product Details MAN2B1 ELISA Kit

Target: MAN2B1 (Mannosidase, Alpha, Class 2B, Member 1 (MAN2B1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Mannosidase Alpha Class 2B Member 1 (MAN2B1). No significant cross-reactivity or interference between Mannosidase Alpha Class 2B Member 1 (MAN2B1) and analogues was observed.

Sensitivity: 14.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosidase Alpha Class 2B Member 1 (MAN2B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannosidase Alpha Class 2B Member 1 (MAN2B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannosidase Alpha Class 2B Member 1 (MAN2B1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannosidase Alpha Class 2B Member 1 (MAN2B1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MAN2B1

Target: MAN2B1 (Mannosidase, Alpha, Class 2B, Member 1 (MAN2B1))

Alternative Name: Mannosidase Alpha Class 2B Member 1 (MAN2B1 Products)

Synonyms: AW107687 ELISA Kit, LAMAN ELISA Kit, Laman ELISA Kit, Manb ELISA Kit, MANB ELISA Kit, mannosidase alpha class 2B member 1 ELISA Kit, mannosidase, alpha, class 2B, member 1 ELISA Kit, mannosidase 2, alpha B1 ELISA Kit, MAN2B1 ELISA Kit, man2b1 ELISA Kit, Man2b1 ELISA Kit

Background:

Gene Name: Mannosidase Alpha Class 2B Member 1

Gene Aliases: MANB, LAMAN, Lysosomal alpha-mannosidase, Lysosomal acid alpha-mannosidase, Mannosidase alpha-B

Gene ID: 4125

UniProt: O00754