Major Basic Protein CLIA Kit
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- Target See all Major Basic Protein products
- Major Basic Protein
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Reactivity
- Mouse
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 40 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Major Basic Protein (MBP). No significant cross-reactivity or interference between Major Basic Protein (MBP) and analogues was observed.
- Sensitivity
- 0.03 ng/mL
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Major Basic Protein ELISA Kit
Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Major Basic Protein ELISA KitReactivity: Human Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Major Basic Protein (MBP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Major Basic Protein (MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Major Basic Protein (MBP) level in the sample or standard.,
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Major Basic Protein (MBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Major Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all Major Basic Protein products
- Major Basic Protein
- Abstract
- Major Basic Protein Products
- Synonyms
- MBP CLIA Kit, mMBP CLIA Kit, mMBP-1 CLIA Kit, myelin basic protein CLIA Kit, proteoglycan 2, bone marrow CLIA Kit, MBP CLIA Kit, Prg2 CLIA Kit
- Background
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Gene Name: Major Basic Protein
Gene Aliases: PRG2, BMPG, Proteoglycan 2, Bone Marrow,Natural Killer Cell Activator, Eosinophil Granule Major Basic Protein, Proteoglycan 2,Bone Marrow, Pregnancy-associated major basic
- UniProt
- Q61878
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