KARS ELISA Kit

Catalog No. ABIN5656570

Product Details KARS ELISA Kit

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Lysyl tRNA Synthetase (KARS). No significant cross-reactivity or interference between Lysyl tRNA Synthetase (KARS) and analogues was observed.

Sensitivity: 0.117 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl tRNA Synthetase (KARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl tRNA Synthetase (KARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl tRNA Synthetase (KARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysyl tRNA Synthetase (KARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for KARS

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Alternative Name: Lysyl tRNA Synthetase (KARS Products)

Synonyms: CG12141 ELISA Kit, Dmel\\CG12141 ELISA Kit, KRS ELISA Kit, LysRS ELISA Kit, cb530 ELISA Kit, wu:fa16h02 ELISA Kit, zgc:92483 ELISA Kit, kars ELISA Kit, MGC53345 ELISA Kit, kars2 ELISA Kit, krs ELISA Kit, krs-1 ELISA Kit, KARS ELISA Kit, Tb06.28P18.190 ELISA Kit, CMTRIB ELISA Kit, DFNB89 ELISA Kit, KARS1 ELISA Kit, KARS2 ELISA Kit, AA589550 ELISA Kit, AL024334 ELISA Kit, AL033315 ELISA Kit, AL033367 ELISA Kit, D8Ertd698e ELISA Kit, D8Wsu108e ELISA Kit, mKIAA0070 ELISA Kit, Lysyl-tRNA synthetase ELISA Kit, lysyl-tRNA synthetase ELISA Kit, lysyl-tRNA synthetase S homeolog ELISA Kit, lysyl-tRNA synthetase L homeolog ELISA Kit, LysRS ELISA Kit, kars ELISA Kit, kars.S ELISA Kit, kars.L ELISA Kit, Bm1_16500 ELISA Kit, lysS ELISA Kit, Taci_1118 ELISA Kit, Tpau_1737 ELISA Kit, Arch_0261 ELISA Kit, Trad_2960 ELISA Kit, Spirs_3699 ELISA Kit, KARS ELISA Kit, LOC100280510 ELISA Kit, Tb927.6.1510 ELISA Kit, Kars ELISA Kit

Background:

Gene Name: Lysyl tRNA Synthetase

Gene Aliases: KARS2, LysRS, Lysine tRNA Ligase

Gene ID: 3735

UniProt: Q15046