LAMP2 ELISA Kit

Catalog No. ABIN5656551

Product Details LAMP2 ELISA Kit

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Lysosomal Associated Membrane Protein 2 (LAMP2). No significant cross-reactivity or interference between Lysosomal Associated Membrane Protein 2 (LAMP2) and analogues was observed.

Sensitivity: 24.9 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysosomal Associated Membrane Protein 2 (LAMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysosomal Associated Membrane Protein 2 (LAMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysosomal Associated Membrane Protein 2 (LAMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysosomal Associated Membrane Protein 2 (LAMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysosomal Associated Membrane Protein 2 (LAMP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysosomal Associated Membrane Protein 2 (LAMP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Alternative Name: Lysosomal Associated Membrane Protein 2 (LAMP2 Products)

Synonyms: CD107b ELISA Kit, LAMP-2 ELISA Kit, LAMPB ELISA Kit, LGP110 ELISA Kit, Lamp-2 ELISA Kit, Lamp-2a ELISA Kit, Lamp-2b ELISA Kit, Lamp-2c ELISA Kit, Mac3 ELISA Kit, cd107b ELISA Kit, lamp-2 ELISA Kit, lampb ELISA Kit, LAMP2 ELISA Kit, lamp2 ELISA Kit, DKFZp459J126 ELISA Kit, LGP-B ELISA Kit, sb:cb587 ELISA Kit, wu:fb14a06 ELISA Kit, zgc:103532 ELISA Kit, lysosomal associated membrane protein 2 ELISA Kit, lysosomal-associated membrane protein 2 ELISA Kit, lysosomal-associated membrane protein 2 S homeolog ELISA Kit, lysosomal membrane glycoprotein 2 ELISA Kit, LAMP2 ELISA Kit, Lamp2 ELISA Kit, lamp2.S ELISA Kit, lamp2 ELISA Kit

Background:

Gene Name: Lysosomal Associated Membrane Protein 2

Gene Aliases: CD107-B, CD107b, LAMP-2C, LAMPB, LGP96, Lysosome-associated membrane glycoprotein 2, CD107 antigen-like family member B

UniProt: P13473

Pathways: Autophagy