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LRP5 ELISA Kit

LRP5 Reactivity: Rat Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5656496
  • Target See all LRP5 ELISA Kits
    LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
    Reactivity
    • 2
    • 2
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.781 ng/mL - 50 ng/mL
    Minimum Detection Limit
    0.781 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein Receptor Related Protein 5 (LRP5). No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Related Protein 5 (LRP5) and analogues was observed.
    Sensitivity
    0.3 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 5 (LRP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Related Protein 5 (LRP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Related Protein 5 (LRP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Density Lipoprotein Receptor Related Protein 5 (LRP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 5 (LRP5) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 5 (LRP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all LRP5 ELISA Kits
    LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
    Alternative Name
    Low Density Lipoprotein Receptor Related Protein 5 (LRP5 Products)
    Synonyms
    Xlrp5 ELISA Kit, bmnd1 ELISA Kit, evr1 ELISA Kit, evr4 ELISA Kit, hbm ELISA Kit, lr3 ELISA Kit, lrp5-A ELISA Kit, lrp7 ELISA Kit, oppg ELISA Kit, ops ELISA Kit, opta1 ELISA Kit, vbch2 ELISA Kit, LRP5 ELISA Kit, LRP6 ELISA Kit, BMND1 ELISA Kit, EVR1 ELISA Kit, EVR4 ELISA Kit, HBM ELISA Kit, LR3 ELISA Kit, LRP7 ELISA Kit, OPPG ELISA Kit, OPS ELISA Kit, OPTA1 ELISA Kit, VBCH2 ELISA Kit, mKIAA4142 ELISA Kit, low-density lipoprotein receptor-related protein 5 ELISA Kit, LDL receptor related protein 5 ELISA Kit, LDL receptor related protein 5 L homeolog ELISA Kit, low density lipoprotein receptor-related protein 5 ELISA Kit, LDL receptor related protein 5 like ELISA Kit, LOC100147353 ELISA Kit, LRP5 ELISA Kit, lrp5.L ELISA Kit, lrp5 ELISA Kit, Lrp5 ELISA Kit, LRP5L ELISA Kit
    Background

    Gene Name: Low Density Lipoprotein Receptor Related Protein 5

    Gene Aliases: HBM, BMND1, EVR1, EVR4, LR3, LRP7, OPPG, OPS, OPTA1, VBCH2, Osteoporosis Pseudoglioma Syndrome, Exudative Vitreoretinopathy 1

    Gene ID
    293649
    UniProt
    B2RYI1
    Pathways
    WNT Signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation
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