LRP2 ELISA Kit

Catalog No. ABIN5656494

Product Details LRP2 ELISA Kit

Target: LRP2 (Low Density Lipoprotein Receptor-Related Protein 2 (LRP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein Receptor Related Protein 2 (LRP2). No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Related Protein 2 (LRP2) and analogues was observed.

Sensitivity: 0.067 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 2 (LRP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Related Protein 2 (LRP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Related Protein 2 (LRP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Density Lipoprotein Receptor Related Protein 2 (LRP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 2 (LRP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 2 (LRP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LRP2

Target: LRP2 (Low Density Lipoprotein Receptor-Related Protein 2 (LRP2))

Alternative Name: Low Density Lipoprotein Receptor Related Protein 2 (LRP2 Products)

Synonyms: im:680590 ELISA Kit, lrp2 ELISA Kit, dbs ELISA Kit, gp330 ELISA Kit, megalin ELISA Kit, DBS ELISA Kit, GP330 ELISA Kit, AI315343 ELISA Kit, AW536255 ELISA Kit, D230004K18Rik ELISA Kit, Gp330 ELISA Kit, Megalin ELISA Kit, LDL receptor related protein 2 ELISA Kit, low density lipoprotein receptor-related protein 2a ELISA Kit, low density lipoprotein receptor-related protein 2 ELISA Kit, low-density lipoprotein receptor-related protein 2 ELISA Kit, LDL receptor related protein 2 L homeolog ELISA Kit, LRP2 ELISA Kit, lrp2a ELISA Kit, LRP2.3 ELISA Kit, lrp2.L ELISA Kit, LOC100183506 ELISA Kit, lrp2 ELISA Kit, Lrp2 ELISA Kit

Background:

Gene Name: Low Density Lipoprotein Receptor Related Protein 2

Gene Aliases: DBS, gp330, Glycoprotein 330, Megalin, Low Density Lipoprotein-Related Protein 2

Gene ID: 4036

UniProt: P98164

Pathways: Metabolism of Steroid Hormones and Vitamin D, Thyroid Hormone Synthesis, Hormone Transport