LDLRAP1 ELISA Kit

Catalog No. ABIN5656489

Product Details LDLRAP1 ELISA Kit

Target: LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1). No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) and analogues was observed.

Sensitivity: 0.067 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LDLRAP1

Target: LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1))

Alternative Name: Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1 Products)

Synonyms: ARH ELISA Kit, ARH1 ELISA Kit, ARH2 ELISA Kit, FHCB1 ELISA Kit, FHCB2 ELISA Kit, AA691260 ELISA Kit, Arh ELISA Kit, Arh1 ELISA Kit, RGD1563417 ELISA Kit, arh ELISA Kit, arh1 ELISA Kit, arh2 ELISA Kit, fhcb1 ELISA Kit, fhcb2 ELISA Kit, xptb ELISA Kit, LDLRAP1 ELISA Kit, ldlrap1 ELISA Kit, sb:cb50 ELISA Kit, zgc:56121 ELISA Kit, zgc:158745 ELISA Kit, low density lipoprotein receptor adaptor protein 1 ELISA Kit, low density lipoprotein receptor adaptor protein 1 L homeolog ELISA Kit, low density lipoprotein receptor adaptor protein 1 S homeolog ELISA Kit, low density lipoprotein receptor adaptor protein 1a ELISA Kit, low density lipoprotein receptor adaptor protein 1b ELISA Kit, LDLRAP1 ELISA Kit, Ldlrap1 ELISA Kit, ldlrap1 ELISA Kit, ldlrap1.L ELISA Kit, ldlrap1.S ELISA Kit, ldlrap1a ELISA Kit, ldlrap1b ELISA Kit

Background:

Gene Name: Low Density Lipoprotein Receptor Adaptor Protein 1

Gene Aliases: ARH1, ARH, ARH2, FHCB1, FHCB2, Autosomal recessive hypercholesterolemia protein

Gene ID: 100017

UniProt: Q8C142

Pathways: Lipid Metabolism