MGLL ELISA Kit

Catalog No. ABIN5656443

Product Details MGLL ELISA Kit

Target: MGLL (Monoglyceride Lipase (MGLL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Lipase, Monoacylglycerol (MGL). No significant cross-reactivity or interference between Lipase, Monoacylglycerol (MGL) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Monoacylglycerol (MGL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Monoacylglycerol (MGL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Monoacylglycerol (MGL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Monoacylglycerol (MGL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Monoacylglycerol (MGL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Monoacylglycerol (MGL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MGLL

Target: MGLL (Monoglyceride Lipase (MGLL))

Alternative Name: Lipase, Monoacylglycerol (MGLL Products)

Synonyms: cb909 ELISA Kit, wu:fc66g01 ELISA Kit, zgc:56561 ELISA Kit, MGC84195 ELISA Kit, MGLL ELISA Kit, mgl ELISA Kit, hu-k5 ELISA Kit, mgll ELISA Kit, HU-K5 ELISA Kit, HUK5 ELISA Kit, MAGL ELISA Kit, MGL ELISA Kit, AA589436 ELISA Kit, Magl ELISA Kit, Mgl ELISA Kit, monoglyceride lipase ELISA Kit, monoglyceride lipase L homeolog ELISA Kit, 13L protein ELISA Kit, Monoglyceride lipase ELISA Kit, mgll ELISA Kit, MGLL ELISA Kit, mgll.L ELISA Kit, 13L ELISA Kit, YMTVg13L ELISA Kit, EHI_044210 ELISA Kit, EHI_045330 ELISA Kit, TATV_DAH68_039 ELISA Kit, Mgll ELISA Kit

Background:

Gene Name: Lipase, Monoacylglycerol

Gene Aliases: HU-K5, MGLL, MAGL, Monoglyceride Lipase, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase

Gene ID: 11343

UniProt: Q99685

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Lipid Metabolism