LIM Domain Binding 1 Protein ELISA Kit

Catalog No. ABIN5656421

Product Details LIM Domain Binding 1 Protein ELISA Kit

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of LIM Domain Binding Protein 1 (LDB1). No significant cross-reactivity or interference between LIM Domain Binding Protein 1 (LDB1) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM Domain Binding Protein 1 (LDB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM Domain Binding Protein 1 (LDB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM Domain Binding Protein 1 (LDB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIM Domain Binding Protein 1 (LDB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIM Domain Binding Protein 1 (LDB1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIM Domain Binding Protein 1 (LDB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LIM Domain Binding 1 Protein

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Alternative Name: LIM Domain Binding Protein 1 (LDB1 Products)

Synonyms: CLIM2 ELISA Kit, NLI ELISA Kit, chi ELISA Kit, clim2 ELISA Kit, nli ELISA Kit, xldb1 ELISA Kit, ldb1 ELISA Kit, LDB1 ELISA Kit, ldb4 ELISA Kit, ldb4b ELISA Kit, LIM domain binding 1 ELISA Kit, LIM domain binding 1 L homeolog ELISA Kit, LIM-domain binding 1b ELISA Kit, LIM domain binding protein 1 ELISA Kit, LIM domain binding 1a ELISA Kit, Ldb1 ELISA Kit, LDB1 ELISA Kit, ldb1.L ELISA Kit, ldb1b ELISA Kit, xldb1 ELISA Kit, ldb1a ELISA Kit

Background:

Gene Name: LIM Domain Binding Protein 1

Gene Aliases: CLIM2, NLI, Carboxy Terminal LIM Domain Protein 2, LIM domain-binding factor CLIM2, Nuclear LIM interactor

Gene ID: 8861

UniProt: Q86U70

Pathways: Stem Cell Maintenance, Chromatin Binding