LIMS1 ELISA Kit

Catalog No. ABIN5656419

Product Details LIMS1 ELISA Kit

Target: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). No significant cross-reactivity or interference between LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LIMS1

Target: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Alternative Name: LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1 Products)

Synonyms: PINCH ELISA Kit, PINCH-1 ELISA Kit, PINCH1 ELISA Kit, Pinch1 ELISA Kit, RGD1560732 ELISA Kit, LIMS3 ELISA Kit, LIMS3L ELISA Kit, 2310016J22Rik ELISA Kit, 4921524A02Rik ELISA Kit, AI507642 ELISA Kit, AU021743 ELISA Kit, AW551584 ELISA Kit, C430041B13Rik ELISA Kit, Lims1l ELISA Kit, LIM zinc finger domain containing 1 ELISA Kit, LIM and senescent cell antigen-like domains 1 ELISA Kit, LIMS1 ELISA Kit, Lims1 ELISA Kit

Background:

Gene Name: LIM And Senescent Cell Antigen Like Domains Protein 1

Gene Aliases: PINCH, PINCH1, Particularly interesting new Cys-His protein 1, Renal carcinoma antigen NY-REN-48

Gene ID: 3987

UniProt: P48059