LEFTY1 CLIA Kit

Catalog No. ABIN5656349

Product Details LEFTY1 CLIA Kit

Target: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 1 (LEFTY1). No significant cross-reactivity or interference between Left/Right Determination Factor 1 (LEFTY1) and analogues was observed.

Sensitivity: 4.77 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Left/Right Determination Factor 1 (LEFTY1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Alternative Name: Left/Right Determination Factor 1 (LEFTY1 Products)

Synonyms: atv CLIA Kit, cb73 CLIA Kit, antivin CLIA Kit, ik:tdsubc_2b12 CLIA Kit, xx:tdsubc_2b12 CLIA Kit, LEFTY1 CLIA Kit, AI450052 CLIA Kit, Ebaf CLIA Kit, Leftb CLIA Kit, Stra3 CLIA Kit, Tgfb4 CLIA Kit, lefty CLIA Kit, lefty-1 CLIA Kit, RGD1561867 CLIA Kit, LEFTB CLIA Kit, LEFTYB CLIA Kit, lefty1 CLIA Kit, signaling molecule lefty1 CLIA Kit, left-right determination factor 1 CLIA Kit, left right determination factor 1 CLIA Kit, lft1 CLIA Kit, lefty1 CLIA Kit, LOC699924 CLIA Kit, LEFTY1 CLIA Kit, LOC100408003 CLIA Kit, LOC100597378 CLIA Kit, Lefty1 CLIA Kit

Background:

Gene Name: Left/Right Determination Factor 1

Gene Aliases: LEFTY-B, LEFTYB, LEFT-B, LEFTB, Left Right Determination Factor B

Gene ID: 498299

UniProt: D4A670