LMAN2 ELISA Kit

Catalog No. ABIN5656344

Product Details LMAN2 ELISA Kit

Target: LMAN2 (Lectin, Mannose-Binding 2 (LMAN2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Lectin, Mannose Binding 2 (LMAN2). No significant cross-reactivity or interference between Lectin, Mannose Binding 2 (LMAN2) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lectin, Mannose Binding 2 (LMAN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lectin, Mannose Binding 2 (LMAN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lectin, Mannose Binding 2 (LMAN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lectin, Mannose Binding 2 (LMAN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lectin, Mannose Binding 2 (LMAN2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lectin, Mannose Binding 2 (LMAN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LMAN2

Target: LMAN2 (Lectin, Mannose-Binding 2 (LMAN2))

Alternative Name: Lectin, Mannose Binding 2 (LMAN2 Products)

Synonyms: Lman2 ELISA Kit, wu:fb16f04 ELISA Kit, zgc:158761 ELISA Kit, C5orf8 ELISA Kit, GP36B ELISA Kit, VIP36 ELISA Kit, 1110003H06Rik ELISA Kit, 1300009F09Rik ELISA Kit, AA408240 ELISA Kit, AL023023 ELISA Kit, AU040819 ELISA Kit, Vip36 ELISA Kit, lectin, mannose binding 2 ELISA Kit, lectin, mannose-binding 2 ELISA Kit, LMAN2 ELISA Kit, lman2 ELISA Kit, Lman2 ELISA Kit

Background:

Gene Name: Lectin, Mannose Binding 2

Gene Aliases: GP36B, VIP36, C5orf8, Vesicular integral-membrane protein VIP36, Glycoprotein GP36b, Vesicular integral-membrane protein 36

Gene ID: 10960

UniProt: Q12907

Pathways: SARS-CoV-2 Protein Interactome