Lamin A/C ELISA Kit

Catalog No. ABIN5656297

Product Details Lamin A/C ELISA Kit

Target: Lamin A/C (LMNA)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Lamin A/C (LMNA). No significant cross-reactivity or interference between Lamin A/C (LMNA) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lamin A/C (LMNA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lamin A/C (LMNA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lamin A/C (LMNA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lamin A/C (LMNA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lamin A/C (LMNA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lamin A/C (LMNA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin A/C (LMNA Products)

Synonyms: CDCD1 ELISA Kit, CDDC ELISA Kit, CMD1A ELISA Kit, CMT2B1 ELISA Kit, EMD2 ELISA Kit, FPL ELISA Kit, FPLD ELISA Kit, FPLD2 ELISA Kit, HGPS ELISA Kit, IDC ELISA Kit, LDP1 ELISA Kit, LFP ELISA Kit, LGMD1B ELISA Kit, LMN1 ELISA Kit, LMNC ELISA Kit, LMNL1 ELISA Kit, PRO1 ELISA Kit, Dhe ELISA Kit, 2459 ELISA Kit, 74/76 ELISA Kit, CG6944 ELISA Kit, D5 ELISA Kit, DM[[O]] ELISA Kit, Dm ELISA Kit, Dm(0) ELISA Kit, Dm0 ELISA Kit, Dm1 ELISA Kit, Dm2 ELISA Kit, DmLamin ELISA Kit, DmO ELISA Kit, Dm[[0]] ELISA Kit, Dm[[1]] ELISA Kit, Dm[[2]] ELISA Kit, Dm[[mit]] ELISA Kit, Dm[[o]] ELISA Kit, Dmel\\CG6944 ELISA Kit, Dmo ELISA Kit, LAM ELISA Kit, Lam Dm[[0]] ELISA Kit, Lam(Dm0) ELISA Kit, LamDm[[0]] ELISA Kit, LamDm[[o]] ELISA Kit, Lam[[Dm0]] ELISA Kit, Lamin Dm[[0]] ELISA Kit, jf27 ELISA Kit, l(2)04643 ELISA Kit, l(2)25Ec ELISA Kit, l(2)gdh-7 ELISA Kit, l(2)gdh7 ELISA Kit, l(2)jf27 ELISA Kit, lam ELISA Kit, lamDm0 ELISA Kit, lamDm[[0]] ELISA Kit, lamin ELISA Kit, lamin Dm0 ELISA Kit, lamin Dm[[0]] ELISA Kit, misg ELISA Kit, nlam ELISA Kit, cb948 ELISA Kit, fk66d12 ELISA Kit, wu:fk66d12 ELISA Kit, lmna-A ELISA Kit, fpl ELISA Kit, idc ELISA Kit, lfp ELISA Kit, cddc ELISA Kit, emd2 ELISA Kit, fpld ELISA Kit, hgps ELISA Kit, ldp1 ELISA Kit, lmn1 ELISA Kit, lmnc ELISA Kit, pro1 ELISA Kit, cdcd1 ELISA Kit, cmd1a ELISA Kit, cmt2b1 ELISA Kit, lgmd1b ELISA Kit, Lamin-A ELISA Kit, lamin-L(III)-like ELISA Kit, lamin A/C ELISA Kit, lamin A ELISA Kit, Lamin ELISA Kit, lamin A/C L homeolog ELISA Kit, lamin-L(III)-like ELISA Kit, LMNA ELISA Kit, Lmna ELISA Kit, Lam ELISA Kit, lmna ELISA Kit, lmna.L ELISA Kit, LMINA ELISA Kit

Background:

Gene Name: Lamin A/C

Gene Aliases: CDCD1, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, PRO1, Cardiomyopathy Dilated 1A, Limb Girdle Muscular Dystrophy 1B, Progeria 1

Gene ID: 4000

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus