LACTB ELISA Kit

Catalog No. ABIN5656269

Product Details LACTB ELISA Kit

Target: LACTB (Lactamase, beta (LACTB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Lactamase Beta (LACTb). No significant cross-reactivity or interference between Lactamase Beta (LACTb) and analogues was observed.

Sensitivity: 0.122 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactamase Beta (LACTb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactamase Beta (LACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactamase Beta (LACTb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactamase Beta (LACTb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactamase Beta (LACTb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactamase Beta (LACTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LACTB

Target: LACTB (Lactamase, beta (LACTB))

Alternative Name: Lactamase Beta (LACTB Products)

Synonyms: BA2507 ELISA Kit, PSPTO2834 ELISA Kit, PSPTO3594 ELISA Kit, G24 ELISA Kit, MRPL56 ELISA Kit, LACT-1 ELISA Kit, Lact1 ELISA Kit, Mrpl56 ELISA Kit, zgc:110419 ELISA Kit, beta-lactamase ELISA Kit, class C beta-lactamase AmpC ELISA Kit, metal-dependent hydrolase ELISA Kit, lactamase beta ELISA Kit, Beta-lactamase ELISA Kit, lactamase, beta ELISA Kit, metallo-beta-lactamase domain containing 2 ELISA Kit, Beta lactamase ELISA Kit, bla1 ELISA Kit, ampC ELISA Kit, PSPTO_2834 ELISA Kit, GSU1378 ELISA Kit, MCA2220 ELISA Kit, CJE0344 ELISA Kit, LACTB ELISA Kit, Rmar_1357 ELISA Kit, MGYG_07395 ELISA Kit, ML5_3452 ELISA Kit, bla ELISA Kit, Lactb ELISA Kit, lactb ELISA Kit, MBLAC2 ELISA Kit, blaKPC-2 ELISA Kit

Background:

Gene Name: Lactamase Beta

Gene Aliases: G24, MRPL56, Mitochondrial Ribosomal Protein L56, Serine beta-lactamase-like protein LACTB, mitochondrial

Gene ID: 114294

UniProt: P83111