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Kynurenic Acid ELISA Kit

KYNA Reactivity: Various Species Colorimetric Competition ELISA 24.69 ng/mL - 2000 ng/mL Plasma, Serum
Catalog No. ABIN5656259
  • Target See all Kynurenic Acid (KYNA) products
    Kynurenic Acid (KYNA)
    Reactivity
    Various Species
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    24.69 ng/mL - 2000 ng/mL
    Minimum Detection Limit
    24.69 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Kynurenic Acid (KYNA). No significant cross-reactivity or interference between Kynurenic Acid (KYNA) and analogues was observed.
    Sensitivity
    9.24 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 h
    Plate
    Pre-coated
    Protocol
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Kynurenic Acid (KYNA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Kynurenic Acid (KYNA) and unlabeled Kynurenic Acid (KYNA) (Standards or samples) with the pre-coated antibody specific to Kynurenic Acid (KYNA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Kynurenic Acid (KYNA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Kynurenic Acid (KYNA) in the sample.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kynurenic Acid (KYNA) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kynurenic Acid (KYNA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Kynurenic Acid (KYNA) products
    Kynurenic Acid (KYNA)
    Alternative Name
    Kynurenic Acid (KYNA Products)
    Target Type
    Chemical
    Background

    Gene Name: Kynurenic Acid

    Gene Aliases: Kinurenic acid, Kynuronic acid, Kuinurenic acid, Transtorine

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