WWC1 ELISA Kit

Catalog No. ABIN5656211

Product Details WWC1 ELISA Kit

Target: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Kidney And Brain Protein (KIBRA). No significant cross-reactivity or interference between Kidney And Brain Protein (KIBRA) and analogues was observed.

Sensitivity: 30 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kidney And Brain Protein (KIBRA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kidney And Brain Protein (KIBRA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kidney And Brain Protein (KIBRA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kidney And Brain Protein (KIBRA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney And Brain Protein (KIBRA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney And Brain Protein (KIBRA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for WWC1

Target: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Alternative Name: Kidney And Brain Protein (WWC1 Products)

Synonyms: CG12600 ELISA Kit, CG33967 ELISA Kit, CG7552 ELISA Kit, Dmel\\CG33967 ELISA Kit, Kibra ELISA Kit, ORE-2 ELISA Kit, RE26350 ELISA Kit, kib ELISA Kit, l(3)02404 ELISA Kit, l(3)j5C3 ELISA Kit, l(3)neo42 ELISA Kit, HBEBP3 ELISA Kit, HBEBP36 ELISA Kit, KIBRA ELISA Kit, AA408228 ELISA Kit, AU017197 ELISA Kit, BC037006 ELISA Kit, mKIAA0869 ELISA Kit, CG33967 gene product from transcript CG33967-RB ELISA Kit, WW and C2 domain containing 1 ELISA Kit, WW, C2 and coiled-coil domain containing 1 ELISA Kit, kibra ELISA Kit, WWC1 ELISA Kit, Wwc1 ELISA Kit

Background:

Gene Name: Kidney And Brain Protein

Gene Aliases: WWC1, WW Domain Containing Protein 1, WW,C2 And Coiled-Coil Domain Containing 1, HBeAg-binding protein 3

Gene ID: 211652

UniProt: Q5SXA9