KRT81 ELISA Kit

Catalog No. ABIN5656201

Product Details KRT81 ELISA Kit

Target: KRT81 (Keratin 81 (KRT81))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 81 (KRT81). No significant cross-reactivity or interference between Keratin 81 (KRT81) and analogues was observed.

Sensitivity: 0.053 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 81 (KRT81). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 81 (KRT81). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 81 (KRT81), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 81 (KRT81) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 81 (KRT81) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 81 (KRT81) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for KRT81

Target: KRT81 (Keratin 81 (KRT81))

Alternative Name: Keratin 81 (KRT81 Products)

Synonyms: KRT81 ELISA Kit, KRTHB5 ELISA Kit, HB1 ELISA Kit, Hb-1 ELISA Kit, KRTHB1 ELISA Kit, MLN137 ELISA Kit, ghHkb1 ELISA Kit, hHAKB2-1 ELISA Kit, AA589625 ELISA Kit, Krt2-19 ELISA Kit, keratin 81 ELISA Kit, keratin, type II cuticular Hb1 ELISA Kit, keratin 85 ELISA Kit, KRT81 ELISA Kit, LOC100012075 ELISA Kit, LOC100092864 ELISA Kit, LOC100394162 ELISA Kit, LOC100481102 ELISA Kit, LOC100526784 ELISA Kit, KRT85 ELISA Kit, Krt81 ELISA Kit

Background:

Gene Name: Keratin 81

Gene Aliases: KRTHB1, Hb-1, K81, MLN137, Keratin,Hair,Basic 1, Hard Keratin Type II 1, Keratin, type II cuticular Hb1, Metastatic lymph node 137 gene protein

Gene ID: 3887

UniProt: Q14533