KRT6A ELISA Kit

Catalog No. ABIN5656198

Product Details KRT6A ELISA Kit

Target: KRT6A (Keratin 6A (KRT6A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 6A (KRT6A). No significant cross-reactivity or interference between Keratin 6A (KRT6A) and analogues was observed.

Sensitivity: 6.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 6A (KRT6A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 6A (KRT6A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 6A (KRT6A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 6A (KRT6A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 6A (KRT6A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 6A (KRT6A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for KRT6A

Target: KRT6A (Keratin 6A (KRT6A))

Alternative Name: Keratin 6A (KRT6A Products)

Synonyms: CK6A ELISA Kit, CK6C ELISA Kit, CK6D ELISA Kit, K6A ELISA Kit, K6C ELISA Kit, K6D ELISA Kit, KRT6C ELISA Kit, KRT6D ELISA Kit, 2310016L08Rik ELISA Kit, Krt2-6a ELISA Kit, Krt2-6c ELISA Kit, MK6a ELISA Kit, mK6[a] ELISA Kit, CK-6A ELISA Kit, Krt6a ELISA Kit, KRT6A ELISA Kit, keratin 6A ELISA Kit, similar to keratin complex 2, basic, gene 6a ELISA Kit, keratin, type II cytoskeletal 6A ELISA Kit, KRT6A ELISA Kit, Krt6a ELISA Kit, LOC683313 ELISA Kit, LOC101112304 ELISA Kit

Background:

Gene Name: Keratin 6A

Gene Aliases: K6A, KRT6C, KRT6D, CK6C, K6C, CK6D, K6D, Keratin 6C, Keratin 6D, Keratin, type II cytoskeletal 6A, Type-II keratin Kb6

Gene ID: 3853

UniProt: P02538