JKAMP ELISA Kit

Catalog No. ABIN5656133

Product Details JKAMP ELISA Kit

Target: JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of JNK1/MAPK8 Associated Membrane Protein (JKAMP). No significant cross-reactivity or interference between JNK1/MAPK8 Associated Membrane Protein (JKAMP) and analogues was observed.

Sensitivity: 0.61 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to JNK1/MAPK8 Associated Membrane Protein (JKAMP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to JNK1/MAPK8 Associated Membrane Protein (JKAMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain JNK1/MAPK8 Associated Membrane Protein (JKAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of JNK1/MAPK8 Associated Membrane Protein (JKAMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level JNK1/MAPK8 Associated Membrane Protein (JKAMP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level JNK1/MAPK8 Associated Membrane Protein (JKAMP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for JKAMP

Target: JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))

Alternative Name: JNK1/MAPK8 Associated Membrane Protein (JKAMP Products)

Synonyms: C14orf100 ELISA Kit, HSPC213 ELISA Kit, HSPC327 ELISA Kit, JAMP ELISA Kit, 1200003C05Rik ELISA Kit, 2310047L11Rik ELISA Kit, Jamp ELISA Kit, RGD1308917 ELISA Kit, JNK1/MAPK8 associated membrane protein ELISA Kit, JNK1/MAPK8-associated membrane protein ELISA Kit, JKAMP ELISA Kit, Jkamp ELISA Kit

Background:

Gene Name: JNK1/MAPK8 Associated Membrane Protein

Gene Aliases: JAMP, C14orf100, Jun N-Terminal Kinase 1-Associated Membrane Protein, Medulloblastoma antigen MU-MB-50.4