JAK2 ELISA Kit

Catalog No. ABIN5656130

Product Details JAK2 ELISA Kit

Target: JAK2 (Janus Kinase 2 (JAK2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Janus Kinase 2 (JAK2). No significant cross-reactivity or interference between Janus Kinase 2 (JAK2) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Janus Kinase 2 (JAK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Janus Kinase 2 (JAK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Janus Kinase 2 (JAK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Janus Kinase 2 (JAK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Janus Kinase 2 (JAK2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Janus Kinase 2 (JAK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: Janus Kinase 2 (JAK2 Products)

Synonyms: JAK2bCA ELISA Kit, jak2 ELISA Kit, JTK10 ELISA Kit, THCYT3 ELISA Kit, AI504024 ELISA Kit, C81284 ELISA Kit, Fd17 ELISA Kit, JAK-2 ELISA Kit, Janus kinase 2b ELISA Kit, Janus kinase 2 ELISA Kit, jak2b ELISA Kit, JAK2 ELISA Kit, Jak2 ELISA Kit

Background:

Gene Name: Janus Kinase 2

Gene Aliases: JTK10, Tyrosine-protein kinase JAK2

Gene ID: 24514

UniProt: Q62689

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response