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IDH1 ELISA Kit

IDH1 Reactivity: Human Colorimetric Sandwich ELISA 12.5 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5656121
  • Target See all IDH1 ELISA Kits
    IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
    Reactivity
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    12.5 ng/mL - 200 ng/mL
    Minimum Detection Limit
    12.5 ng/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Isocitrate Dehydrogenase 1, Soluble (IDH1). No significant cross-reactivity or interference between Isocitrate Dehydrogenase 1, Soluble (IDH1) and analogues was observed.
    Sensitivity
    4.7 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    1 - 2 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Standards or samples and HRP-labeled detection antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Isocitrate Dehydrogenase 1, Soluble (IDH1), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Isocitrate Dehydrogenase 1, Soluble (IDH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all IDH1 ELISA Kits
    IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
    Alternative Name
    Isocitrate Dehydrogenase 1, Soluble (IDH1 Products)
    Synonyms
    IDCD ELISA Kit, IDH ELISA Kit, IDP ELISA Kit, IDPC ELISA Kit, PICD ELISA Kit, NADP-CICDH ELISA Kit, AI314845 ELISA Kit, AI788952 ELISA Kit, E030024J03Rik ELISA Kit, Id-1 ELISA Kit, Idh-1 ELISA Kit, Idpc ELISA Kit, cb876 ELISA Kit, fm90e09 ELISA Kit, im:7143416 ELISA Kit, wu:fm90e09 ELISA Kit, F23E12.180 ELISA Kit, F23E12_180 ELISA Kit, IDH-I ELISA Kit, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 ELISA Kit, isocitrate dehydrogenase 1 ELISA Kit, isocitrate dehydrogenase I ELISA Kit, isocitrate dehydrogenase (NADP(+)) 1, cytosolic ELISA Kit, isocitrate dehydrogenase 1 (NADP+), soluble ELISA Kit, isocitrate dehydrogenase 1 (NADP+) L homeolog ELISA Kit, isocitrate dehydrogenase 1 ELISA Kit, IDH1 ELISA Kit, Idh1 ELISA Kit, idh1.L ELISA Kit, idh1 ELISA Kit
    Background

    Gene Name: Isocitrate Dehydrogenase 1, Soluble

    Gene Aliases: PICD, IDP, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, Cytosolic NADP-isocitrate dehydrogenase

    Gene ID
    3417
    UniProt
    O75874
    Pathways
    Warburg Effect
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