INA ELISA Kit

Catalog No. ABIN5656104

Product Details INA ELISA Kit

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Internexin Neuronal Intermediate Filament Protein Alpha (INa). No significant cross-reactivity or interference between Internexin Neuronal Intermediate Filament Protein Alpha (INa) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Internexin Neuronal Intermediate Filament Protein Alpha (INa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Internexin Neuronal Intermediate Filament Protein Alpha (INa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Internexin Neuronal Intermediate Filament Protein Alpha (INa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Internexin Neuronal Intermediate Filament Protein Alpha (INa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Internexin Neuronal Intermediate Filament Protein Alpha (INa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Internexin Neuronal Intermediate Filament Protein Alpha (INa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for INA

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Alternative Name: Internexin Neuronal Intermediate Filament Protein Alpha (INA Products)

Synonyms: NEF5 ELISA Kit, NF-66 ELISA Kit, TXBP-1 ELISA Kit, Inexa ELISA Kit, Intlaa ELISA Kit, Nf66 ELISA Kit, AV028420 ELISA Kit, NF66 ELISA Kit, ina-A ELISA Kit, xefiltin ELISA Kit, ina-a ELISA Kit, inexa ELISA Kit, MGC82496 ELISA Kit, INA ELISA Kit, si:ch211-67n3.6 ELISA Kit, nef5 ELISA Kit, xina ELISA Kit, nf-66 ELISA Kit, txbp-1 ELISA Kit, MGC69277 ELISA Kit, gefiltin ELISA Kit, zgc:110516 ELISA Kit, internexin neuronal intermediate filament protein alpha ELISA Kit, internexin neuronal intermediate filament protein, alpha ELISA Kit, internexin neuronal intermediate filament protein alpha S homeolog ELISA Kit, low molecular weight neuronal intermediate filament S homeolog ELISA Kit, internexin neuronal intermediate filament protein, alpha a ELISA Kit, internexin neuronal intermediate filament protein, alpha b ELISA Kit, INA ELISA Kit, Ina ELISA Kit, ina.S ELISA Kit, nif.S ELISA Kit, inaa ELISA Kit, ina ELISA Kit, inab ELISA Kit

Background:

Gene Name: Internexin Neuronal Intermediate Filament Protein Alpha

Gene Aliases: NEF5, NF-66, 66 kDa neurofilament protein, Neurofilament-66, Neurofilament 5