IL11RA ELISA Kit

Catalog No. ABIN5655749

Product Details IL11RA ELISA Kit

Target: IL11RA (Interleukin 11 Receptor, alpha (IL11RA))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 11 Receptor Alpha (IL11Ra). No significant cross-reactivity or interference between Interleukin 11 Receptor Alpha (IL11Ra) and analogues was observed.

Sensitivity: 0.064 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 11 Receptor Alpha (IL11Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 11 Receptor Alpha (IL11Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 11 Receptor Alpha (IL11Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 11 Receptor Alpha (IL11Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 11 Receptor Alpha (IL11Ra) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 11 Receptor Alpha (IL11Ra) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for IL11RA

Target: IL11RA (Interleukin 11 Receptor, alpha (IL11RA))

Alternative Name: Interleukin 11 Receptor Alpha (IL11RA Products)

Synonyms: CRSDA ELISA Kit, fi26e06 ELISA Kit, il-11ra ELISA Kit, wu:fi26e06 ELISA Kit, AI314697 ELISA Kit, GP130 ELISA Kit, Il-11ra ELISA Kit, Il11ra ELISA Kit, Il11ra2 ELISA Kit, NR1 ELISA Kit, interleukin 11 receptor subunit alpha ELISA Kit, interleukin 11 receptor, alpha ELISA Kit, interleukin 11 receptor subunit alpha 1 ELISA Kit, interleukin 11 receptor, alpha chain 1 ELISA Kit, IL11RA ELISA Kit, il11ra ELISA Kit, Il11ra1 ELISA Kit, Il11ra ELISA Kit

Background:

Gene Name: Interleukin 11 Receptor Alpha

Gene ID: 245983

UniProt: Q99MF4

Pathways: JAK-STAT Signaling, Growth Factor Binding