IL10RB ELISA Kit

Catalog No. ABIN5655739

Product Details IL10RB ELISA Kit

Target: IL10RB (Interleukin 10 Receptor, beta (IL10RB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 10 Receptor Beta (IL10Rb). No significant cross-reactivity or interference between Interleukin 10 Receptor Beta (IL10Rb) and analogues was observed.

Sensitivity: 31 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 Receptor Beta (IL10Rb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 Receptor Beta (IL10Rb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 Receptor Beta (IL10Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10 Receptor Beta (IL10Rb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 Receptor Beta (IL10Rb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 Receptor Beta (IL10Rb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for IL10RB

Target: IL10RB (Interleukin 10 Receptor, beta (IL10RB))

Alternative Name: Interleukin 10 Receptor Beta (IL10RB Products)

Synonyms: IL10R2 ELISA Kit, IL10RB ELISA Kit, il10r2 ELISA Kit, CDW210B ELISA Kit, CRF2-4 ELISA Kit, CRFB4 ELISA Kit, D21S58 ELISA Kit, D21S66 ELISA Kit, IL-10R2 ELISA Kit, 6620401D04Rik ELISA Kit, AI528744 ELISA Kit, Crfb4 ELISA Kit, D16H21S58 ELISA Kit, D21S58h ELISA Kit, Il10r2 ELISA Kit, RGD1560373 ELISA Kit, interleukin 10 receptor subunit beta ELISA Kit, interleukin 10 receptor subunit beta S homeolog ELISA Kit, interleukin-10 receptor subunit beta ELISA Kit, interleukin 10 receptor, beta ELISA Kit, IL10RB ELISA Kit, il10rb.S ELISA Kit, LOC473966 ELISA Kit, LOC478404 ELISA Kit, il10rb ELISA Kit, Il10rb ELISA Kit

Background:

Gene Name: Interleukin 10 Receptor Beta

Gene Aliases: CDW210b, CD210-B, IL10R-B, IL10-RB, CRF2-4, CRFB4, IL-10R2, Interleukin-10 receptor subunit 2

Gene ID: 3588

UniProt: Q08334