IL1F9 ELISA Kit

Catalog No. ABIN5655676

Product Details IL1F9 ELISA Kit

Target: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.81 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Epsilon (IL1e). No significant cross-reactivity or interference between Interleukin 1 Epsilon (IL1e) and analogues was observed.

Sensitivity: 2.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Epsilon (IL1e). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Epsilon (IL1e). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Epsilon (IL1e), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Epsilon (IL1e) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Epsilon (IL1e) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Epsilon (IL1e) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for IL1F9

Target: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Alternative Name: Interleukin 1 Epsilon (IL1F9 Products)

Synonyms: IL-1F9 ELISA Kit, IL-1H1 ELISA Kit, IL-1RP2 ELISA Kit, IL1E ELISA Kit, IL1F9 ELISA Kit, IL1H1 ELISA Kit, IL1RP2 ELISA Kit, Il36g ELISA Kit, Il1f9 ELISA Kit, RGD1563019 ELISA Kit, IL36G ELISA Kit, interleukin 36 gamma ELISA Kit, interleukin 1 family, member 9 ELISA Kit, interleukin 36, gamma ELISA Kit, interleukin-36 gamma ELISA Kit, IL36G ELISA Kit, Il1f9 ELISA Kit, Il36g ELISA Kit, LOC100065031 ELISA Kit

Background:

Gene Name: Interleukin 1 Epsilon

Gene Aliases: IL1F6, IL36a, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), Interleukin 36, Alpha, Interleukin 1 Family, Member 6

Gene ID: 27179

UniProt: Q9UHA7