CXCL11 ELISA Kit

Catalog No. ABIN5655623

Product Details CXCL11 ELISA Kit

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). No significant cross-reactivity or interference between Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) and analogues was observed.

Sensitivity: 12.4 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Inducible T-Cell Alpha Chemoattractant (ITaC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CXCL11

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Alternative Name: Interferon Inducible T-Cell Alpha Chemoattractant (CXCL11 Products)

Synonyms: H174 ELISA Kit, I-TAC ELISA Kit, IP-9 ELISA Kit, IP9 ELISA Kit, SCYB11 ELISA Kit, SCYB9B ELISA Kit, b-R1 ELISA Kit, Cxc11 ELISA Kit, I-tac ELISA Kit, Ip9 ELISA Kit, Itac ELISA Kit, Scyb11 ELISA Kit, Scyb9b ELISA Kit, betaR1 ELISA Kit, C-X-C motif chemokine ligand 11 ELISA Kit, chemokine (C-X-C motif) ligand 11 ELISA Kit, CXCL11 ELISA Kit, Cxcl11 ELISA Kit

Background:

Gene Name: Interferon Inducible T-Cell Alpha Chemoattractant

Gene Aliases: CXCL11, I-TAC, IP9, SCYB11, SCYB9B, B-R1, Chemokine(C-X-C-Motif)ligand 11, Small Inducible Cytokine Subfamily B Member 11, Interferon gamma-inducible protein 9

Gene ID: 56066

UniProt: Q9JHH5