Integrin beta 3 ELISA Kit

Catalog No. ABIN5655503

Product Details Integrin beta 3 ELISA Kit

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Integrin Beta 3 (ITGb3). No significant cross-reactivity or interference between Integrin Beta 3 (ITGb3) and analogues was observed.

Sensitivity: 0.31 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 3 (ITGb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 3 (ITGb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 3 (ITGb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Beta 3 (ITGb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Beta 3 (ITGb3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Beta 3 (ITGb3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: Integrin Beta 3 (ITGB3 Products)

Synonyms: BDPLT16 ELISA Kit, BDPLT2 ELISA Kit, CD61 ELISA Kit, GP3A ELISA Kit, GPIIIa ELISA Kit, GT ELISA Kit, INGRB3 ELISA Kit, integrin ELISA Kit, INTB3 ELISA Kit, GPIIIA ELISA Kit, cd61 ELISA Kit, gp3a ELISA Kit, itgb3 ELISA Kit, itgb3.2 ELISA Kit, itgb3.1 ELISA Kit, integrin subunit beta 3 ELISA Kit, integrin beta 3 ELISA Kit, integrin subunit beta 3 L homeolog ELISA Kit, integrin beta 3a ELISA Kit, integrin beta 3b ELISA Kit, ITGB3 ELISA Kit, Itgb3 ELISA Kit, itgb3.L ELISA Kit, itgb3a ELISA Kit, itgb3b ELISA Kit

Background:

Gene Name: Integrin Beta 3

Gene Aliases: CD61, ITG-B3, GP3A, GP3-A, GpIIIa, Platelet Membrane Glycoprotein IIIa

Gene ID: 3690

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex