ITGA3 ELISA Kit

Catalog No. ABIN5655489

Product Details ITGA3 ELISA Kit

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Integrin Alpha 3 (ITGa3). No significant cross-reactivity or interference between Integrin Alpha 3 (ITGa3) and analogues was observed.

Sensitivity: 0.118 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 3 (ITGa3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 3 (ITGa3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 3 (ITGa3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha 3 (ITGa3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Alpha 3 (ITGa3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Alpha 3 (ITGa3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ITGA3

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Alternative Name: Integrin Alpha 3 (ITGA3 Products)

Synonyms: CD49C ELISA Kit, GAP-B3 ELISA Kit, GAPB3 ELISA Kit, ILNEB ELISA Kit, MSK18 ELISA Kit, VCA-2 ELISA Kit, VL3A ELISA Kit, VLA3a ELISA Kit, ITGA3 ELISA Kit, AA407068 ELISA Kit, integrin subunit alpha 3 ELISA Kit, integrin subunit alpha 3 S homeolog ELISA Kit, integrin alpha 3 ELISA Kit, ITGA3 ELISA Kit, itga3.S ELISA Kit, Itga3 ELISA Kit

Background:

Gene Name: Integrin Alpha 3

Gene Aliases: CD49c, CD49-C, ITG-A3, GAP-B3, GAPB3, MSK18, VCA-2, VL3A, VLA3a, FRP-2, Galactoprotein B3, Alpha 3 Subunit Of VLA-3 Receptor, CD49 antigen-like family member C

Gene ID: 16400

UniProt: Q62470

Pathways: CXCR4-mediated Signaling Events, Integrin Complex