IGF2BP3 CLIA Kit

Catalog No. ABIN5655414

Product Details IGF2BP3 CLIA Kit

Target: IGF2BP3 (Insulin-Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 312.5 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312.5 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). No significant cross-reactivity or interference between Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) and analogues was observed.

Sensitivity: 9.91 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: IGF2BP3 (Insulin-Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3))

Alternative Name: Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3 Products)

Synonyms: CT98 CLIA Kit, IMP-3 CLIA Kit, IMP3 CLIA Kit, KOC1 CLIA Kit, VICKZ3 CLIA Kit, Ab2-255 CLIA Kit, RGD1306512 CLIA Kit, imp3 CLIA Kit, koc1 CLIA Kit, vera CLIA Kit, imp-3 CLIA Kit, vg1rbp CLIA Kit, vickz3 CLIA Kit, 2610101N11Rik CLIA Kit, AA522010 CLIA Kit, AL022933 CLIA Kit, AU045931 CLIA Kit, Koc13 CLIA Kit, Neilsen CLIA Kit, mimp3 CLIA Kit, dvr1rbp CLIA Kit, wu:fb79c12 CLIA Kit, wu:fe06h09 CLIA Kit, zVg1RBP CLIA Kit, B3 CLIA Kit, IGF2 mRNA-binding protein 3-B CLIA Kit, IMP CLIA Kit, IMP-3-B CLIA Kit, Vg1 RBP-B CLIA Kit, Vg1-RBP CLIA Kit, igf2bp3 CLIA Kit, igf2bp3-a CLIA Kit, igf2bp3-b CLIA Kit, IGF2 mRNA-binding protein 3-A CLIA Kit, IMP-3-A CLIA Kit, Vg1 RBP-A CLIA Kit, vera-A CLIA Kit, insulin like growth factor 2 mRNA binding protein 3 CLIA Kit, insulin-like growth factor 2 mRNA binding protein 3 CLIA Kit, insulin like growth factor 2 mRNA binding protein 3 S homeolog CLIA Kit, insulin like growth factor 2 mRNA binding protein 3 L homeolog CLIA Kit, IGF2BP3 CLIA Kit, Igf2bp3 CLIA Kit, igf2bp3 CLIA Kit, igf2bp3.S CLIA Kit, igf2bp3.L CLIA Kit

Background:

Gene Name: Insulin Like Growth Factor 2 mRNA Binding Protein 3

Gene Aliases: MP-3, IMP3, KOC1, VICKZ3, CT98, IGF II mRNA Binding Protein 3, Cancer/Testis Antigen 98, KH domain-containing protein overexpressed in cancer

Gene ID: 10643

UniProt: O00425