IDE ELISA Kit

Catalog No. ABIN5655389

Product Details IDE ELISA Kit

Target: IDE (Insulin-Degrading Enzyme (IDE))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Degrading Enzyme (IDE). No significant cross-reactivity or interference between Insulin Degrading Enzyme (IDE) and analogues was observed.

Sensitivity: 0.125 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Degrading Enzyme (IDE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Degrading Enzyme (IDE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Degrading Enzyme (IDE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Degrading Enzyme (IDE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Degrading Enzyme (IDE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Degrading Enzyme (IDE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for IDE

Target: IDE (Insulin-Degrading Enzyme (IDE))

Alternative Name: Insulin Degrading Enzyme (IDE Products)

Synonyms: zgc:162603 ELISA Kit, IDE ELISA Kit, 1300012G03Rik ELISA Kit, 4833415K22Rik ELISA Kit, AA675336 ELISA Kit, AI507533 ELISA Kit, INSDEGM ELISA Kit, INSULYSIN ELISA Kit, insulin degrading enzyme ELISA Kit, insulin-degrading enzyme ELISA Kit, metallopeptidase ELISA Kit, IDE ELISA Kit, ide ELISA Kit, LOC591315 ELISA Kit, CC1G_01115 ELISA Kit, Bm1_26615 ELISA Kit, PTRG_10875 ELISA Kit, SJAG_01430 ELISA Kit, EBI_21700 ELISA Kit, VDBG_06702 ELISA Kit, LOAG_08082 ELISA Kit, PGTG_04982 ELISA Kit, Tsp_00424 ELISA Kit, Ide ELISA Kit

Background:

Gene Name: Insulin Degrading Enzyme

Gene Aliases: Insulysin, Insulin Protease, Abeta-degrading protease, Insulinase

Pathways: SARS-CoV-2 Protein Interactome