INPP5D ELISA Kit

Catalog No. ABIN5655369

Product Details INPP5D ELISA Kit

Target: INPP5D (Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Inositol Polyphosphate-5-Phosphatase 145 kDa (INPP5D). No significant cross-reactivity or interference between Inositol Polyphosphate-5-Phosphatase 145 kDa (INPP5D) and analogues was observed.

Sensitivity: 13.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol Polyphosphate-5-Phosphatase 145 kDa (INPP5D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inositol Polyphosphate-5-Phosphatase 145 kDa (INPP5D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inositol Polyphosphate-5-Phosphatase 145 kDa (INPP5D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inositol Polyphosphate-5-Phosphatase 145 kDa (INPP5D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for INPP5D

Target: INPP5D (Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D))

Alternative Name: Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D Products)

Synonyms: INPP5D ELISA Kit, 256.t00004 ELISA Kit, 13.t00027 ELISA Kit, ship ELISA Kit, ship1 ELISA Kit, AI323613 ELISA Kit, SHIP ELISA Kit, SHIP-1 ELISA Kit, SHIP1 ELISA Kit, s-SHIP ELISA Kit, SIP-145 ELISA Kit, hp51CN ELISA Kit, inositol polyphosphate-5-phosphatase D ELISA Kit, phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 ELISA Kit, inositol polyphosphate 5-phosphatase ELISA Kit, inositol polyphosphate-5-phosphatase D L homeolog ELISA Kit, INPP5D ELISA Kit, LOC100446045 ELISA Kit, inpp5d ELISA Kit, EHI_159880 ELISA Kit, EHI_153490 ELISA Kit, inpp5d.L ELISA Kit, LOC100380693 ELISA Kit, Inpp5d ELISA Kit

Background:

Gene Name: Inositol Polyphosphate-5-Phosphatase 145 kDa

Gene Aliases: SHIP, SHIP1, SIP-145, hp51CN, Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatase 1, SH2 domain-containing inositol 5'-phosphatase 1

Pathways: TCR Signaling, BCR Signaling, Warburg Effect