IgG2a ELISA Kit
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- Target See all IgG2a products
- IgG2a
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Detection Range
- 246.91 ng/mL - 20000 ng/mL
- Minimum Detection Limit
- 246.91 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G2a (IgG2a). No significant cross-reactivity or interference between Immunoglobulin G2a (IgG2a) and analogues was observed.
- Sensitivity
- 96.3 ng/mL
- Featured
- Discover our best selling IgG2a ELISA Kit
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IgG2a ELISA Kit
Reactivity: Mouse Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgG2a ELISA KitReactivity: Guinea Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
IgG2a ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG2a ELISA KitReactivity: Rat Colorimetric Competition ELISA 246.91 ng/mL - 20000 ng/mL Plasma, Serum
IgG2a ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma, Serum
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 h
- Plate
- Pre-coated
- Protocol
- The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Immunoglobulin G2a (IgG2a) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G2a (IgG2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G2a (IgG2a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G2a (IgG2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all IgG2a products
- IgG2a
- Alternative Name
- Immunoglobulin G2a (IgG2a Products)
- Synonyms
- gamma-2a immunoglobulin heavy chain ELISA Kit, IgG-2a ELISA Kit
- Target Type
- Antibody
- Background
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Gene Name: Immunoglobulin G2a
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