HMBS ELISA Kit

Catalog No. ABIN5655222

Product Details HMBS ELISA Kit

Target: HMBS (Hydroxymethylbilane Synthase (HMBS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Hydroxymethylbilane Synthase (HMBS). No significant cross-reactivity or interference between Hydroxymethylbilane Synthase (HMBS) and analogues was observed.

Sensitivity: 0.26 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydroxymethylbilane Synthase (HMBS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hydroxymethylbilane Synthase (HMBS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydroxymethylbilane Synthase (HMBS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxymethylbilane Synthase (HMBS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hydroxymethylbilane Synthase (HMBS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hydroxymethylbilane Synthase (HMBS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HMBS

Target: HMBS (Hydroxymethylbilane Synthase (HMBS))

Alternative Name: Hydroxymethylbilane Synthase (HMBS Products)

Synonyms: PBG-D ELISA Kit, PBGD ELISA Kit, PORC ELISA Kit, UPS ELISA Kit, URO-S ELISA Kit, hemC ELISA Kit, pbg-d ELISA Kit, pbgd ELISA Kit, ups ELISA Kit, SPAC806.01 ELISA Kit, DDBDRAFT_0186148 ELISA Kit, DDBDRAFT_0231417 ELISA Kit, DDB_0186148 ELISA Kit, DDB_0231417 ELISA Kit, Hmbs ELISA Kit, NV50236 ELISA Kit, T25658 ELISA Kit, Ups ELISA Kit, Uros1 ELISA Kit, hmbs ELISA Kit, zgc:64128 ELISA Kit, hmbsl ELISA Kit, id:ibd5004 ELISA Kit, im:7140060 ELISA Kit, zgc:110690 ELISA Kit, hydroxymethylbilane synthase ELISA Kit, hydroxymethylbilane synthase S homeolog ELISA Kit, hydroxymethylbilane synthase L homeolog ELISA Kit, hydroxymethylbilane synthase (predicted) ELISA Kit, Hydroxymethylbilane synthase ELISA Kit, hydroxymethylbilane synthase a ELISA Kit, hydroxymethylbilane synthase, b ELISA Kit, HMBS ELISA Kit, Hmbs ELISA Kit, hmbs ELISA Kit, hmbs.2.S ELISA Kit, hmbs.L ELISA Kit, CNC02250 ELISA Kit, hem3 ELISA Kit, hemC ELISA Kit, Trad_0335 ELISA Kit, Plabr_2100 ELISA Kit, Sgly_3113 ELISA Kit, SMLT_RS19660 ELISA Kit, PMI_RS16580 ELISA Kit, hmbsa ELISA Kit, hmbsb ELISA Kit

Background:

Gene Name: Hydroxymethylbilane Synthase

Gene Aliases: PBG-D, PBGD, UPS, Hydroxymethylbilane Synthase, Uroporphyrinogen I Synthase, Porphobilinogen deaminase, Pre-uroporphyrinogen synthase

Gene ID: 3145

UniProt: P08397