HGD ELISA Kit

Catalog No. ABIN5655196

Product Details HGD ELISA Kit

Target: HGD (Homogentisate 1,2-Dioxygenase (HGD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Homogentisate-1,2-Dioxygenase (HGD). No significant cross-reactivity or interference between Homogentisate-1,2-Dioxygenase (HGD) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homogentisate-1,2-Dioxygenase (HGD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Homogentisate-1,2-Dioxygenase (HGD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Homogentisate-1,2-Dioxygenase (HGD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Homogentisate-1,2-Dioxygenase (HGD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Homogentisate-1,2-Dioxygenase (HGD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Homogentisate-1,2-Dioxygenase (HGD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HGD

Target: HGD (Homogentisate 1,2-Dioxygenase (HGD))

Alternative Name: Homogentisate-1,2-Dioxygenase (HGD Products)

Synonyms: CG4779 ELISA Kit, Dmel\\CG4779 ELISA Kit, PSPTO3551 ELISA Kit, DDBDRAFT_0185702 ELISA Kit, DDBDRAFT_0191461 ELISA Kit, DDB_0185702 ELISA Kit, DDB_0191461 ELISA Kit, Hgo ELISA Kit, aku ELISA Kit, hgo ELISA Kit, AKU ELISA Kit, HGO ELISA Kit, homogentisate 1,2-dioxygenase ELISA Kit, Homogentisate 1,2-dioxygenase ELISA Kit, homogentisate 1, 2-dioxygenase ELISA Kit, homogentisate 1,2-dioxygenase L homeolog ELISA Kit, hgo ELISA Kit, HGD ELISA Kit, hmgA ELISA Kit, Shewana3_2481 ELISA Kit, hgd ELISA Kit, BC1002_0545 ELISA Kit, BC1003_0502 ELISA Kit, BC1001_0489 ELISA Kit, Sinme_2995 ELISA Kit, Sphch_0441 ELISA Kit, Hgd ELISA Kit, hgd.L ELISA Kit

Background:

Gene Name: Homogentisate-1,2-Dioxygenase

Gene Aliases: AKU, HGO, Homogentisic Acid Oxidase, Homogentisicase, Homogentisate oxygenase

Gene ID: 3081

UniProt: Q93099