Histone H4 ELISA Kit

Catalog No. ABIN5655183

Product Details Histone H4 ELISA Kit

Target: Histone H4

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Histone H4 (H4). No significant cross-reactivity or interference between Histone H4 (H4) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H4 (H4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone H4 (H4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H4 (H4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone H4 (H4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone H4 (H4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone H4 (H4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Histone H4

Target: Histone H4

Abstract: Histone H4 Products

Synonyms: hist1h4l ELISA Kit, H1ft ELISA Kit, Hist4 ELISA Kit, Hist4h4 ELISA Kit, H4 ELISA Kit, X04652 ELISA Kit, FO108 ELISA Kit, H4/n ELISA Kit, H4F2 ELISA Kit, H4FN ELISA Kit, HIST2H4 ELISA Kit, H4C13 ELISA Kit, H4C7 ELISA Kit, F8N16.2 ELISA Kit, F8N16_2 ELISA Kit, histone H4 ELISA Kit, BcDNA:RH52884 ELISA Kit, CG3379 ELISA Kit, Dmel\CG3379 ELISA Kit, H4r ELISA Kit, His4-88CD ELISA Kit, His4R ELISA Kit, histone cluster 1, H4a L homeolog ELISA Kit, histone cluster 1 H4 family member B ELISA Kit, histone cluster 2, H4 ELISA Kit, histone cluster 2 H4 family member a ELISA Kit, histone H4 ELISA Kit, Histone H4 replacement ELISA Kit, Histone H4 ELISA Kit, hist1h4a.L ELISA Kit, Hist1h4b ELISA Kit, Hist2h4 ELISA Kit, HIST2H4A ELISA Kit, H4 ELISA Kit, H4C14 ELISA Kit, HIS4 ELISA Kit, HIST2H4 ELISA Kit, His4r ELISA Kit, his-38 ELISA Kit

Background:

Gene Name: Histone H4