Histone H2A ELISA Kit

Catalog No. ABIN5655179

Product Details Histone H2A ELISA Kit

Target: Histone H2A

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Histone H2A (H2A). No significant cross-reactivity or interference between Histone H2A (H2A) and analogues was observed.

Sensitivity: 0.124 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H2A (H2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone H2A (H2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H2A (H2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone H2A (H2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone H2A (H2A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone H2A (H2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Histone H2A

Target: Histone H2A

Abstract: Histone H2A Products

Synonyms: 5499 ELISA Kit, CG5499 ELISA Kit, Dmel\\CG5499 ELISA Kit, H2A ELISA Kit, H2A.F/Z ELISA Kit, H2A.X ELISA Kit, H2A.Z ELISA Kit, H2AV ELISA Kit, H2AV_DROM ELISA Kit, H2AX ELISA Kit, H2AZ ELISA Kit, H2Av ELISA Kit, H2AvD ELISA Kit, H2a.V ELISA Kit, H2av ELISA Kit, H2avD ELISA Kit, HIS ELISA Kit, HIS2AV ELISA Kit, HIS2AVD ELISA Kit, His ELISA Kit, His2 ELISA Kit, His2AV ELISA Kit, His2AvD ELISA Kit, HisH2Av ELISA Kit, Hist ELISA Kit, Hist2av ELISA Kit, gamma-H2Av ELISA Kit, gamma-HIS2AV ELISA Kit, gamma-His2Av ELISA Kit, gammaH2Av ELISA Kit, h2AvD ELISA Kit, his ELISA Kit, l(3)05146 ELISA Kit, l(3)810 ELISA Kit, l(3)97Dd ELISA Kit, l(3)L1602 ELISA Kit, DDBDRAFT_0205974 ELISA Kit, DDBDRAFT_0216271 ELISA Kit, DDB_0205974 ELISA Kit, DDB_0216271 ELISA Kit, RGD1564767 ELISA Kit, H2a-221 ELISA Kit, H2A-III ELISA Kit, HIST1H2AA ELISA Kit, Histone H2A variant ELISA Kit, putative histone H2A ELISA Kit, histone H2A ELISA Kit, histone cluster 1 H2A family member F ELISA Kit, histone H2A-like ELISA Kit, histone cluster 1, H2af ELISA Kit, histone2A1 ELISA Kit, histone cluster 1, H2A, III ELISA Kit, histone cluster 1, H2ac ELISA Kit, His2Av ELISA Kit, LMJF_17_0280 ELISA Kit, H2AX ELISA Kit, Hist1h2af ELISA Kit, LOC100425378 ELISA Kit, his2a1 ELISA Kit, HIST1H2A3 ELISA Kit, HIST1H2AC ELISA Kit, h2a-A ELISA Kit

Background:

Gene Name: Histone H2A