HDAC6 ELISA Kit

Catalog No. ABIN5655175

Product Details HDAC6 ELISA Kit

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Histone Deacetylase 6 (HDAC6). No significant cross-reactivity or interference between Histone Deacetylase 6 (HDAC6) and analogues was observed.

Sensitivity: 0.118 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 6 (HDAC6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Deacetylase 6 (HDAC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Deacetylase 6 (HDAC6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone Deacetylase 6 (HDAC6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Deacetylase 6 (HDAC6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Deacetylase 6 (HDAC6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HDAC6

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Alternative Name: Histone Deacetylase 6 (HDAC6 Products)

Synonyms: HD6 ELISA Kit, Hd6 ELISA Kit, Hdac5 ELISA Kit, Sfc6 ELISA Kit, mHDA2 ELISA Kit, CG6170 ELISA Kit, DHDAC2 ELISA Kit, DmHDAC2 ELISA Kit, Dmel\\CG6170 ELISA Kit, HDAC ELISA Kit, HDAC2 ELISA Kit, dHDAC2 ELISA Kit, dHDAC6 ELISA Kit, dmHDA404 ELISA Kit, hdac6 ELISA Kit, MGC53140 ELISA Kit, wu:fc31d02 ELISA Kit, dsim_GLEANR_17355 ELISA Kit, DsimGD17207 ELISA Kit, GD17207 ELISA Kit, HDAC6 ELISA Kit, ATHDA6 ELISA Kit, AXE1 ELISA Kit, HISTONE DEACETYLASE 6 ELISA Kit, MDC12.7 ELISA Kit, MDC12_7 ELISA Kit, RNA-MEDIATED TRANSCRIPTIONAL SILENCING 1 ELISA Kit, RPD3B ELISA Kit, RTS1 ELISA Kit, SIL1 ELISA Kit, histone deacetylase 6 ELISA Kit, histone deacetylase 6 ELISA Kit, Histone deacetylase 6 ELISA Kit, histone deacetylase 6 L homeolog ELISA Kit, GD17207 gene product from transcript GD17207-RB ELISA Kit, Histone DeAcetylase ELISA Kit, HDAC6 ELISA Kit, Hdac6 ELISA Kit, hdac6.L ELISA Kit, hdac6 ELISA Kit, Dsim\HDAC6 ELISA Kit, PTRG_03035 ELISA Kit, HDA6 ELISA Kit, hda-6 ELISA Kit

Background:

Gene Name: Histone Deacetylase 6

Gene Aliases: HD6, JM21

Gene ID: 10013

UniProt: Q9UBN7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling