HDAC4 ELISA Kit

Catalog No. ABIN5655173

Product Details HDAC4 ELISA Kit

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Histone Deacetylase 4 (HDAC4). No significant cross-reactivity or interference between Histone Deacetylase 4 (HDAC4) and analogues was observed.

Sensitivity: 0.129 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 4 (HDAC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Deacetylase 4 (HDAC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Deacetylase 4 (HDAC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone Deacetylase 4 (HDAC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Deacetylase 4 (HDAC4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Deacetylase 4 (HDAC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HDAC4

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Alternative Name: Histone Deacetylase 4 (HDAC4 Products)

Synonyms: CG1770 ELISA Kit, DHDAC4 ELISA Kit, Dmel\\CG1770 ELISA Kit, GC1770 ELISA Kit, HDAC ELISA Kit, HDAC4a ELISA Kit, dHDAC4 ELISA Kit, dmHDA405 ELISA Kit, hdac4 ELISA Kit, dana_GLEANR_19966 ELISA Kit, DanaGF18710 ELISA Kit, GF18710 ELISA Kit, HDAC4 ELISA Kit, AHO3 ELISA Kit, BDMR ELISA Kit, HA6116 ELISA Kit, HD4 ELISA Kit, HDAC-4 ELISA Kit, HDAC-A ELISA Kit, HDACA ELISA Kit, 4932408F19Rik ELISA Kit, AI047285 ELISA Kit, wu:fa96d08 ELISA Kit, wu:fc56f08 ELISA Kit, zgc:152701 ELISA Kit, histone deacetylase 4 ELISA Kit, hypothetical protein ELISA Kit, Histone deacetylase 4 ELISA Kit, GF18710 gene product from transcript GF18710-RC ELISA Kit, PTRG_03699 ELISA Kit, PGTG_15512 ELISA Kit, HDAC4 ELISA Kit, Dana\GF18710 ELISA Kit, Hdac4 ELISA Kit, hdac4 ELISA Kit

Background:

Gene Name: Histone Deacetylase 4

Gene Aliases: HD4, HDAC-A, HDACA

Gene ID: 208727

UniProt: Q6NZM9

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process