HDC ELISA Kit

Catalog No. ABIN5655154

Product Details HDC ELISA Kit

Target: HDC (Histidine Decarboxylase (HDC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2.34 ng/mL - 150 ng/mL

Minimum Detection Limit: 2.34 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Histidine Decarboxylase (HDC). No significant cross-reactivity or interference between Histidine Decarboxylase (HDC) and analogues was observed.

Sensitivity: 0.7 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Decarboxylase (HDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Decarboxylase (HDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Decarboxylase (HDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Decarboxylase (HDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histidine Decarboxylase (HDC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histidine Decarboxylase (HDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HDC

Target: HDC (Histidine Decarboxylase (HDC))

Alternative Name: Histidine Decarboxylase (HDC Products)

Synonyms: 4732480P20 ELISA Kit, AW108189 ELISA Kit, Hdc-a ELISA Kit, Hdc-c ELISA Kit, Hdc-e ELISA Kit, Hdc-s ELISA Kit, CG3454 ELISA Kit, Dmel\\CG3454 ELISA Kit, JK910 ELISA Kit, anon-WO0140519.259 ELISA Kit, hdc ELISA Kit, HDC ELISA Kit, LOC100313635 ELISA Kit, histidine decarboxylase ELISA Kit, Histidine decarboxylase ELISA Kit, HDC ELISA Kit, Hdc ELISA Kit, LOC548615 ELISA Kit, hdc ELISA Kit, Desac_2880 ELISA Kit, LOC100313635 ELISA Kit, LOC100533309 ELISA Kit

Background:

Gene Name: Histidine Decarboxylase

Gene ID: 3067

UniProt: P19113