HMGA1 ELISA Kit

Catalog No. ABIN5655127

Product Details HMGA1 ELISA Kit

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of High Mobility Group AT Hook Protein 1 (HMGA1). No significant cross-reactivity or interference between High Mobility Group AT Hook Protein 1 (HMGA1) and analogues was observed.

Sensitivity: 0.124 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group AT Hook Protein 1 (HMGA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group AT Hook Protein 1 (HMGA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group AT Hook Protein 1 (HMGA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group AT Hook Protein 1 (HMGA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group AT Hook Protein 1 (HMGA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group AT Hook Protein 1 (HMGA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HMGA1

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Alternative Name: High Mobility Group AT Hook Protein 1 (HMGA1 Products)

Synonyms: HMG-R ELISA Kit, HMGA1A ELISA Kit, HMGIY ELISA Kit, Hmgi ELISA Kit, Hmgiy ELISA Kit, hmga1 ELISA Kit, hmgiy ELISA Kit, wu:fb12b06 ELISA Kit, wu:fb18f11 ELISA Kit, wu:fc19f01 ELISA Kit, wu:fi38b05 ELISA Kit, zgc:85677 ELISA Kit, AL023995 ELISA Kit, Hmga1a ELISA Kit, Hmga1b ELISA Kit, Hmgy ELISA Kit, HMG-I(Y) ELISA Kit, HMG-Y ELISA Kit, HMGA1 ELISA Kit, zgc:153948 ELISA Kit, high mobility group AT-hook 1 ELISA Kit, high mobility group protein HMG-I/HMG-Y ELISA Kit, high mobility group AT-hook 1a ELISA Kit, high mobility group AT-hook 1b ELISA Kit, HMGA1 ELISA Kit, Hmga1 ELISA Kit, TSC_c24220 ELISA Kit, hmga1a ELISA Kit, LOC705105 ELISA Kit, hmga1b ELISA Kit

Background:

Gene Name: High Mobility Group AT Hook Protein 1

Gene Aliases: HMG-R, HMGIY, High mobility group protein A1, High mobility group protein R, High mobility group protein HMG-I/HMG-Y

Gene ID: 3159

UniProt: P17096

Pathways: Nuclear Hormone Receptor Binding, Autophagy